Levamisole acts primarily as a nicotinic acetylcholine receptor agonist on the nerve ganglia of susceptible nematodes. By mimicking acetylcholine, it causes continuous stimulation of the parasite's nervous system. This results in the rapid and irreversible spastic paralysis of the worm musculature. The paralyzed worms are unable to maintain their position and are expelled from the gastrointestinal tract by normal peristaltic action. Additionally, Levamisole exhibits an immunostimulatory effect, enhancing the animal's natural defenses post-treatment.